Journal: Biomarker Research

Article Title: Single-cell and spatial transcriptome analysis reveals the potential therapeutic targets for testicular sex cord-stromal cell tumor

doi: 10.1186/s40364-026-00924-0

Figure Lengend Snippet: Disordered cellular communication in TSCST. ( A ) Bar plot showing the number of inferred interactions in the leading edge and the tumor core. ( B ) Bar plot showing the interaction strength in the leading edge and the tumor core. ( C ) Circle plots display ligand-receptor interaction weights between distinct cellular components in the leading edge and the tumor core. Colors distinguish cell types. The circle size is proportional to the number of cells in each cluster, and the edge width represents communication probability. ( D ) Dot plots show the increased ligand-receptor interactions on the leading edge. ( E ) Dot plots showing the increased ligand-receptor interactions in the tumor core. ( F ) Dot plots show increased signaling in macrophages to tumor and Sertoli cells. ( G ) Feature plots of APP-CD74 by ST in Patient 3 and Patient 4. ( H ) Feature plots of MDK-NCL by ST in Patient 3 and Patient 4. ( I ) Mapped SPP and MK signaling pathways in ST, colored by Seurat clusters. ( J ) Violin plots showing the expression level of APP-CD74 genes across the cell types in the leading edge and the tumor core, respectively. The y-axis shows the normalized read count. ( K ) Representative immunofluorescence images showing expression of CTNNB1 and CD74 (n = 4). Nuclei were stained with DAPI. ( L ) Relative mRNA expression of MRC1 and ARG1 (M2-type macrophage markers) and IL1B (M1-type macrophage marker) in macrophages treated with APP. ( M ) Relative mRNA expression of MMP2 (a marker associated with phagocytic function in macrophages) in macrophages treated with APP. The P value was calculated by Welch’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: THP-1-induced macrophages treated with APP protein (MedChemExpress, Shanghai, Catalogue number: HY- P72834 ) were collected, and total RNA was extracted using TRIzol Reagent (Invitrogen, 15596018CN, USA).

Techniques: Protein-Protein interactions, Expressing, Immunofluorescence, Staining, Marker

Journal: Neurotherapeutics

Article Title: Discovery of an APP-selective BACE1 inhibitor for Alzheimer's disease

doi: 10.1016/j.neurot.2025.e00610

Figure Lengend Snippet: Inhibitory activity and brain permeability of FAH65E(-) . Shown are (A) dose-response curves for FAH65 racemate and the FAH65E(+) and (-) enantiomers in the P5-P5′ assay and (B) sAPPβ and (C) Aβ1-42 in CHO-7W cells after treatment with increasing concentrations of FAH65 racemate and enantiomers. Legend in B also applies to C. Data graphed as the mean and SEM. (D) FAH65E(-) (black line) and sAPP β (blue dashed line) levels in brain from ApoE4TR-5XFAD mice after oral delivery of 30 ​mg/kg FAH65E(-) doses are shown. PK-PD study design did not include 0 h timepoint untreated mice but included time points 1, 2, 4 and 6 ​h after last dose on Day 2. N ​= ​3 mice per time point.

Article Snippet: Media was assayed using an AlphaLISA for Aβ1-42 (Perkin Elmer catalog # AL276C), sAPPα (R&D Systems catalog # AF1168 conjugated with Perkin Elmer acceptor beads catalog # 6772001 + 2B3 antibody from IBL catalog # 11088 biotinylated), and sAPPβ (Perkin Elmer AL276-acceptor + IBL catalog # 18957 biotinylated).

Techniques: Activity Assay, Permeability

Journal: Nature Aging

Article Title: Serum amyloid P secreted by bone marrow adipocytes drives skeletal amyloidosis

doi: 10.1038/s43587-025-00924-z

Figure Lengend Snippet: ( a-b ) Immunofluorescence staining of tibial bone sections from WT and APP-PS1 (AD) mice was performed using an anti-perilipin antibody. Representative images of Perilipin + cells in the proximal tibial area are shown in ( a ), and in the distal tibia are shown in ( b ). Scale bars: 500 μm for low magnification and 50 μm for high magnification. n=5.

Article Snippet: Bone sections were blocked in PBS with 3% BSA for 1 h and then stained overnight (>8 h) with individual primary antibodies specific to p19 ARF (Novus, NB200-111, 1:100), γH2AX (Cell Signaling, 20E3, 1:200), perilipin (Cell Signaling, 9349, 1:200) and perilipin (Sigma, P1873, 1:500) and antibodies recognizing human APP (6E10, Novus, NBP2-62566, 1:200) and human Aβ (clone H31L21, Thermo Fisher Scientific 700254, 1:100).

Techniques: Immunofluorescence, Staining

Journal: Nature Aging

Article Title: Serum amyloid P secreted by bone marrow adipocytes drives skeletal amyloidosis

doi: 10.1038/s43587-025-00924-z

Figure Lengend Snippet: Nine-month-old APP-PS1 (AD) mice and WT littermates were used. ( a ) Quantification of hAPP mRNA expression in bone and brain tissues by TaqMan real-time PCR. Data represents the mean ± SD from three independent experiments (n = 3). ( b-d ) Bone and brain tissues were subjected to immunofluorescence staining ( b-c ) and immunohistochemistry (IHC) ( d ) using 6E10 antibody recognizing human APP. Scale bar = 50 μm. Three mice per group (n=3) were used, with three slides prepared per mouse.

Article Snippet: Bone sections were blocked in PBS with 3% BSA for 1 h and then stained overnight (>8 h) with individual primary antibodies specific to p19 ARF (Novus, NB200-111, 1:100), γH2AX (Cell Signaling, 20E3, 1:200), perilipin (Cell Signaling, 9349, 1:200) and perilipin (Sigma, P1873, 1:500) and antibodies recognizing human APP (6E10, Novus, NBP2-62566, 1:200) and human Aβ (clone H31L21, Thermo Fisher Scientific 700254, 1:100).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining, Immunohistochemistry

Journal: Nature Aging

Article Title: Serum amyloid P secreted by bone marrow adipocytes drives skeletal amyloidosis

doi: 10.1038/s43587-025-00924-z

Figure Lengend Snippet: ( a-b ) Bone marrow supernatant was harvested from long bones of APP-PS1 (AD) and wild-type (WT) mice or from aged (24-month-old) and young (4-month-old) mice, and SAP concentrations in the bone marrow were measured. Results are shown in ( a ) for AD vs. WT and ( b ) for old vs. young mice. (n=3). ( c-d ) Bone tissue sections from aged mice were subjected to immunofluorescence staining using antibodies against osteocalcin (OCN) and SAP to assess SAP expression in osteoblasts. Representative images from trabecular ( c ) and cortical ( d ) bone regions demonstrate a lack of SAP signal in OCN+ osteoblasts. Scale bar = 50 μm. n=3. Data are represented as mean ± SEM. **p < 0.01, ***p < 0.001, as determined by unpaired two-tailed Student’s t test.

Article Snippet: Bone sections were blocked in PBS with 3% BSA for 1 h and then stained overnight (>8 h) with individual primary antibodies specific to p19 ARF (Novus, NB200-111, 1:100), γH2AX (Cell Signaling, 20E3, 1:200), perilipin (Cell Signaling, 9349, 1:200) and perilipin (Sigma, P1873, 1:500) and antibodies recognizing human APP (6E10, Novus, NBP2-62566, 1:200) and human Aβ (clone H31L21, Thermo Fisher Scientific 700254, 1:100).

Techniques: Immunofluorescence, Staining, Expressing, Two Tailed Test

Journal: Nature Aging

Article Title: Serum amyloid P secreted by bone marrow adipocytes drives skeletal amyloidosis

doi: 10.1038/s43587-025-00924-z

Figure Lengend Snippet: Peripheral visceral (sub-gonadal) white adipose tissue was collected from 9-month-old APP-PS1 (AD) mice and wild-type (WT) mice as well as from 4- (Young), and 24-month-old mice (Old). ( a-d ) Double-immunofluorescence staining with Perilipin and γH2AX. Representative images of Perilipin + γH2AX + cells are shown in ( a, c ), with quantifications in ( b, d ). (E-H) Double-immunofluorescence staining with Perilipin and p19ARF. Representative images of Perilipin + p19ARF + cells are shown in ( e, g ), with quantifications in ( f, h ). ( i-l ) Double-immunofluorescence staining with antibodies against Perilipin and SAP or amyloid β (H31L21). Representative images of Perilipin + SAP + cells are shown in ( i, j ), and Perilipin + H31L21 + cells in ( k, l ). Scale bar: 50 μm. n = 5. Data are presented as mean ± SEM. Significance: **p < 0.01, ***p < 0.001, **p < 0.0001 as determined by unpaired two-tailed Student’s t -test.

Article Snippet: Bone sections were blocked in PBS with 3% BSA for 1 h and then stained overnight (>8 h) with individual primary antibodies specific to p19 ARF (Novus, NB200-111, 1:100), γH2AX (Cell Signaling, 20E3, 1:200), perilipin (Cell Signaling, 9349, 1:200) and perilipin (Sigma, P1873, 1:500) and antibodies recognizing human APP (6E10, Novus, NBP2-62566, 1:200) and human Aβ (clone H31L21, Thermo Fisher Scientific 700254, 1:100).

Techniques: Double Immunofluorescence Staining, Two Tailed Test